Fig. 3

Nesfatin-1 pre-treatment conspicuously decreased the OA chondrocytes autophagy at 24 h timepoint. A–C The mRNA of Atg5, Atg7 and Beclin1 by qRT-PCR at 24 h timepoint in OA chondrocytes. D Western blot results demonstrated that 10 ng/ml of nesfatin-1 decreased the expression levels of autophagy correlated proteins including Atg5, Atg7, Beclin1 and LC3II/I ratio at 24 h timepoint. Data are expressed as means ± SD of 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 with 0 ng/ml group; nsd means no significant difference