Fig. 2

Effect of PLGA microsphere release solution on NPCs. A Alamarblue was used to detect the proliferation level of nucleus pulposus cells under the release of PLGA microspheres at different time points. B Flow cytometry was used to detect the apoptotic level of nucleus pulposus cells under the release of PLGA microspheres in different groups. C–G RT-qPCR, western blot and immunofluorescence were used to detect the expression levels of phenotypic genes and proteins of nucleus pulposus cells in different groups (n ≥ 3, *p < 0.05, **p < 0.01)