Fig. 3

GPR84 prevents osteoclast formation through inhibiting MAPK pathways. A and B BMMs isolated from bone marrow were cultured in CM from MC-38 cells and transfected with GPR84-overexpressing vectors (GPR84 OE) or vectors. The protein levels of ERK, JNK, p38 MAPK and their phosphorylation of these proteins were determined by western blotting assay and the protein levels were normalized to β-actin (A) and quantification of the protein levels normalized to β-actin (B) (n = 3). C, D Representative TRAP stain images of BMMs were cultured in CM from MC-38 cells, then the cells were transfected with or without GPR84-overexpressing vectors or blank vectors (C) and quantification of osteoclast number (D) (n = 5). Bar represents 50 μm. Significant differences are indicated as *p < 0.05, **p < 0.01 or ***p < 0.001