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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Myricetin alleviated hydrogen peroxide-induced cellular senescence of nucleus pulposus cell through regulating SERPINE1

Fig. 4

Silencing of SERPINE1 regulates HO-induced senescence in NPCs. A Venn diagram analysis of overlapping genes in aging and inflammation-related genes. B qPCR verifies the expression of the top 5 overlapping genes. C The silencing effect of SERPINE1 was detected by qPCR. D SA-β-gal staining was used to detect the level of cellular senescence in two groups of NPCs treated with 100 μM HO for 24 h. E Western blot was used to detect the expression levels of senescence markers (p21, p16) in HO-treated NPCs in si-NC and si-SERPINE1 groups. F The expression levels of IL-6 and IL-8 of NPCs treated with 100 μM HO for 24 h were detected by ELISA. *P < 0.5 VS si-NC group

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X