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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: Inhibition of NUCB2 suppresses the proliferation, migration, and invasion of rheumatoid arthritis synovial fibroblasts from patients with rheumatoid arthritis in vitro

Fig. 2

Effect of NUCB2 silencing on RASF proliferation. A RASFs were stably transfected with sh-NUCB2 NC, sh-NUCB2#1, and sh-NUCB2#2, and the mRNA level of NUCB2 was analyzed using RT-qPCR. B, C The protein level of nesfatin-1 was analyzed using Western blotting. D The growth curves of cells were evaluated by CCK-8 assay after knocking down NUCB2 in RASFs. E, F An EdU assay was performed to evaluate cell proliferation. The samples were imaged at × 200 magnification. Three experiments were performed using RASFs isolated from three different RA patients, and a representative result is shown. **P < 0.01 vs. sh-NUCB2 NC group

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X