Fig. 5

EBLN3P increased MTX-resistance of osteosarcoma cells by enhancing miR-200a-3p/OGT axis. A Upregulation of EBLN3P (EBLN3P OE) increased the invasion ability of MNNG/HOS and MG63 cells. B Column comparison of invaded cells in MNNG/HOS and MG63 cells. **p < 0.01. C and D Effect of blocking OGT on EBLN3P upregulated cells was evaluated. Blocking OGT by OGT-si decreased the resistant effect of EBLN3P on MTX in MNNG/HOS and MG63 cells. *p < 0.05, **p < 0.01, compared with vector; ^#p < 0.05, ^##p < 0.01, compared with EBLN3P OE + OGT-si. E Western blot analyzing the effect of EBLN3P, miR-200a-3p, and OGT on MNNG/HOS cells. Upregulation of EBLN3P leads to increased expression of OGT, Vimentin, and N-cadherin while decreasing the expression of E-cadherin. Further upregulation of miR-200a-3p or knockdown OGT (OGT-si) decreased the expression of OGT, Vimentin, and N-cadherin. *p < 0.05, **p < 0.01, compared with vector; ^#p < 0.05, ^##p < 0.01, compared with EBLN3P OE