Fig. 4

miR-200a-3p increases MTX sensitivity via OGT. A The binding site between miR-200a-3p and OGT. B and C The immunohistochemistry and western analysis of OGT expression in osteosarcoma and adjacent tissues. D The correlation analysis showed that the expression of OGT is negatively correlated with the miR-200a-3p. E and F Upregulation of miR-200a-3p increases the sensitization of osteosarcoma cells to MTX, while upregulation of OGT almost abolished the effect of miR-200a-3p. *p < 0.05, **p < 0.01, compared with miR-200a-3p mimic; ^#p < 0.05, ^##p < 0.01, compared with miR-200a-3p mimic + OGT OE