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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: LncRNA EBLN3P attributes methotrexate resistance in osteosarcoma cells through miR-200a-3p/O-GlcNAc transferase pathway

Fig. 3

EBLN3P sponge miR-200a-3p in MTX resistance osteosarcoma. A The potential binding site of miR-200a-3p with EBLN3P. B The expression of miR-200a-3p was significantly decreased in the osteosarcoma tissue compared to adjacent tissues. C The expression of miR-200a-3p in osteosarcoma patients of GSE65071 and GSE79181 demonstrated significantly downregulated expression in osteosarcoma patients or osteosarcoma patients with relapse. D Knockdown of EBLN3P (EBLN3P-si) increased miR-200a-3p expression in MTX-resistant osteosarcoma cell line MNNG/HOS-MTXr and MG63-MTXr. E The luciferase activity of the WT- EBLN3P sequence was suppressed by the mimic of miR-200a-3p while the activity did not affect the Mut- EBLN3P (mutant EBLN3P) sequence compared with negative control sequence of miR-200a-3p (miR-200a-3p NC). **p < 0.01

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X