Fig. 2

AS IV alleviates apoptosis in NPCs. Cells were incubated with or without AS IV (100 μM) for 2 h, and then treated with 10 ng/ml IL-1β for 24 h. a–d Cleaved-caspase-3, BAX, and BCL-2 levels were evaluated by Western blotting and then quantified. e, f Apoptosis in NPCs was analysed by flow cytometry. Data are expressed as means ± SD. ***P < 0.001 versus control group. ^###P < 0.001, ^##P < 0.01 and ^#P < 0.05 versus IL-1β group