Fig. 8

RUNX2 overexpression impeded the regulatory effect of ASC-338⁺-Exo on ATDC5 cell proliferation and apoptosis. A The cell proliferation of ATDC5 cells was tested by CCK-8 assay. B and C Flow cytometry analysis of apoptosis in ATDC5 cells. ^**P < 0.01 (VS. Blank), ^#P < 0.05 (VS. IL-1β), ^##P < 0.01 (VS. IL-1β), ^&P < 0.05 (VS. IL-1β + ASC-388⁺-Exo), ^$P < 0.05 (VS. IL-1β + RUNX2). ASC-338⁺-Exo, exosomes derived from miR-338-3p mimic-treated ASCs; RUNX2, pcDNA-RUNX2