Fig. 7

RUNX2 overexpression reversed ASC-338⁺-Exo-mediated attenuation of ATDC5 cell inflammation and degradation. A–C The expression levels of PGE2, IL-6, IL-1β, and TNF-αin ATDC5 cell supernatant were detected by ELISA. D Gene expression level of MMP3, MMP13, aggrecan, and Col2a1 in ATDC5 cells was determined by Western blot analysis. β-actin was used as the internal reference gene. I–L Immunofluorescence (IF) was used to test aggrecan and Col2a1 expression in ATDC5 cells. Nuclei were visualized using DAPI. Scale bar: 50 μm. ^**P < 0.01 (VS. Blank), ^#P < 0.05 (VS. IL-1β), ^##P < 0.01 (VS. IL-1β), ^&P < 0.05 (VS. IL-1β + ASC-388⁺-Exo), ^$P < 0.05 (VS. IL-1β + RUNX2). ASC-338⁺-Exo, exosomes derived from miR-338-3p mimic-treated ASCs; RUNX2, pcDNA-RUNX2