Fig. 6

MiR-338-3p derived from ASC-Exos targeted RUNX2 expression in ATDC5 cells. A Alignment of miR-338-3p and the 3′-UTR of RUNX2, a potential miR-338-3p target. B A luciferase reporter carrying the 3’-UTR of wild-type (RUNX2 3′UTR-WT) or mutant (RUNX2 3′UTR-MUT) RUNX2 was introduced into HEK-293T cells along with NC mimic or miR-338-3p mimic. Gene expression level of RUNX2 in ATDC5 cells was determined by RT-qPCR (C and D) and Western blot analysis (D–E, I–J). β-actin was used as the internal reference gene. ^**P < 0.01 (VS. ASC-NC-Exo/vector). ASC-NC-Exo, exosomes derived from NC mimic-treated ASCs; ASC-338⁺-Exo, exosomes derived from miR-338-3p mimic-treated ASCs; RUNX2, pcDNA-RUNX2