Fig. 2
ASC-Exo promoted IL-1β-reduced cell proliferation and inhibited IL-1β-induced cell apoptosis of ATDC5 cells and in delivering miR-338-3p. A–D The expression levels of PGE2, IL-6, IL-1β, and TNF-α in ATDC5 cell supernatant were detected by ELISA. E and F Gene expression level of MMP3 and MMP13 in ATDC5 cells was determined by Western blot analysis. β-actin was used as the internal reference gene. G and H The expression of aggrecan and Col2a1 was assayed by Western blot analysis. β-actin was used as the internal reference gene. I–L Immunofluorescence (IF) was used to test aggrecan and Col2a1 expression in ATDC5 cells. Nuclei were visualized using DAPI. Scale bar: 50 μm.**P < 0.01 (VS. NC), ##P < 0.01 (VS. IL-1β), &P < 0.05 (VS. IL-1β + ASCs), &&P < 0.01 (VS. IL-1β + ASCs). ASCGW4869, ASCs treated with GW4869; ASC-NC−, ASCs treated with NC inhibitor; ASC-338−, ASCs treated with miR-338-3p inhibitor; ASC-NC+, ASCs treated with NC mimic; ASC-338+, ASCs treated with miR-338-3p mimic