Fig. 6

MiR-204-5p constrained RA progression via downregulating YY1 in RA-FLSs. A–I Transfection of miR-NC, miR-204-5p, miR-204-5p + pcDNA, or miR-204-5p + YY1 was performed in RA-FLSs. (A) YY1 level was gauged by western blot. B–G CCK-8 assay, EdU assay, flow cytometry, transwell assay and wound-healing assay were implemented to reveal cell viability, proliferation, apoptosis, invasion and migration, individually. H The protein quantification of cleaved caspase-3 and MMP9 was performed by western blot. I Inflammatory response was measured by ELISA assay. J The impact of the transfection of si-NC, si-circ_0083964, si-circ_0083964 + anti-miR-NC or si-circ_0083964 + anti-miR-204-5p on the expression of YY1 in RA-FLSs was analyzed by western blot. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001