Fig. 4

MiR-204-5p inhibition overturned the effects caused by circ_0083964 knockdown in RA-FLSs. A–I RA-FLSs were transfected with si-NC, si-circ_0083964, si-circ_0083964 + anti-miR-NC or si-circ_0083964 + anti-miR-204-5p. A The expression of miR-204-5p was detected by qRT-PCR. B–G CCK-8 assay, EdU assay, flow cytometry, transwell assay and wound-healing assay were used to assess cell viability, proliferation, apoptosis, invasion and migration, respectively. H The protein levels of cleaved caspase-3 and MMP9 were detected by western blot. I The levels of IL-6 and TNF-α in RA-FLSs were evaluated by ELISA assay. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001