Table 1 Oligonucleotide primers used in real-time PCR
Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
Rat GAPDH | 5′-ACAGCAACAGGGTGGTGGAC-3′ | 5′-TTTGAGGGTGCAGCGAACTT-3′ |
Rat LC3 | 5′-AAAATGGGGCACGGATGAAG-3′ | 5′-GCAGGTCTTCAAAATGCCCA-3′ |
Rat MMP13 | 5′-CCCGAGACCTCATGTTCATCT-3′ | 5′-CTTCTTCTATGAGGCGGGGAT-3′ |
Rat Foxo1 | 5′-CCATGCCTCACACATCTGCC-3′ | 5′-TTAAAATCCAAGGTATCTCCGTCCA-3′ |
Rat SOX9 | 5′-CACAAGAAAGACCACCCCGA-3′ | 5′-TGCACGTCTGTTTTGGGAGT-3′ |
Rat Aggrecan | 5′-TGACTTTCCTCCGTCTACTGTC-3′ | 5′-AGGTCTTCTGTGATCGGTACTC-3′ |
Rat ATG5 | 5′-GTTAGTGAGATTTGGTTTGA-3′ | 5′-ATTTTCTTCTGGAGGGTATT-3′ |
Rat Beclin 1 | 5′-GAGGTACCGACTTGTTCCCT-3′ | 5′-CCTTTCTCCACGTCCATCCT-3′ |
Rat Caspase-3 | 5′-TGGACTGCGGTATTGAGACA-3′ | 5′-GCGCAAAGTGACTGGATGAA-3′ |
Rat SIRT1 | 5′-TGCCATCATGAAGCCAGAGA-3′ | 5′-CATCGCAGTCTCCAAGAAGC-3′ |
Rat BAX | 5′-AAGAAGCTGAGCGAGTGTCT-3′ | 5′-CCAGTTGAAGTTGCCGTCTG-3′ |
Rat Bcl-2 | 5′-TGACTGAGTACCTGAACCGGCATCT-3′ | 5′-GAGACAGCCAGGAGAAATCAAACAGA-3′ |
Homo GAPDH | 5′-TCAAGAAGGTGGTGAAGCAGG-3′ | 5′-TCAAAGGTGGAGGAGTGGGT-3′ |
Homo SIRT1 | 5′-AGCAGATTAGTAGGCGGCTT-3′ | 5′-GACTCTGGCATGTCCCACTA-3′ |
Homo FOXO1 | 5′-TGAAACGAGCAACTATCAAAGAC-3′ | 5′-ATAAAAGAACCAGATGGAGGACT-3′ |