Fig. 6
From: Circ_0136666 aggravates osteosarcoma development through mediating miR-1244/CEP55 axis
CEP55 overexpression rescues the malignant behaviors of circ_0136666-silenced OS cells. A The transfection efficiency of CEP55 plasmid was analyzed by Western blot assay. B–Q OS cells were transfected with si-circ_0136666 alone or together with CEP55 plasmid. B and C Cell proliferation ability was determined by EDU assay. D and E Colony formation assay was carried out to analyze cell proliferation ability. F and G Cell migration ability was assessed using transwell migration assay. H and I Transwell invasion assay was conducted to analyze cell invasion ability. J and K Flow cytometry was utilized to assess cell apoptosis rate. L–O The consumption of glucose and the level of lactate were analyzed using their matching kits. P and Q Glycolysis-associated proteins (HK2 and PKM2) were measured by Western blot assay. ***P < 0.001, ##P < 0.01, ###P < 0.001