Fig. 2

Circ_0136666 silencing suppresses the malignant properties of OS cells. A–J OS cells were transfected with si-NC, si-circ_0136666#1, or si-circ_0136666#2. A RT-qPCR was utilized to analyze circ_0136666 expression in U2OS and SaOS2 cells. B Cell proliferation was assessed by EDU assay. C Cell proliferation ability was assessed via colony formation assay. D and E Transwell assays were performed to assess cell migration and invasion abilities. F The apoptosis rate (FITC positive and PI positive or negative) was analyzed by flow cytometry. G and H The consumption of glucose and the production of lactate were analyzed using their corresponding kits. I and J Western blot assay was performed to measure the protein levels of glycolysis-association markers (HK2 and PKM2) in transfected OS cells. ***P < 0.001