Fig. 5

MAFB was a downstream target of miR-1286. A Binding sites between MAFB 3′UTR and miR-1286 sequences. B, C Dual-luciferase reporter assay was conducted to validate interaction between miR-1286 and MAFB. D, E MAFB mRNA and protein levels were detected by RT-qPCR and western blot after transfection of anti-miR-1286, miR-2186 and corresponding control groups. F–I RT-qPCR and western blot were applied for expression analysis of MAFB in OS tissues (F, G) and SaoS-2/U2OS cells (H, I). J Pearson’s correlation coefficient was performed for linear analysis between MAFB and miR-1286 in OS samples. *P < 0.05