Fig. 2

NEAT1 downregulates miR-338-3p by sponging as ceRNA. A FLSs-RA was transfected with control siRNA or NEAT1 siRNA and miR-338-3p expression was detected by qRT-PCR. B FLSs-RA cell lysates were incubated with biotin-labeled control, sense or antisense NEAT1 probes for pull-down assays. miR-338-3p enrichment was assessed by qRT-PCR. C Luciferase reporter assays were performed by transfection of FLSs-RA with luciferase vectors containing WT- or Mut-NEAT1 and control miRNA or miR-338-3p. Luciferase activity was examined; significant differences between the two groups were identified using the Mann–Whitney U test. Values are means (± SD, n = 3). *p < 0.05, **p < 0.01