Fig. 5

HMGB-1 affects osteoporosis via the inhibition of TLR signaling pathway. a Expression of TLR signaling pathway-related proteins TLR4, MyD88 p-P65 and P65 was detected by western blot. *P < 0.05, vs WT Sham group; ^#P < 0.05, vs WT OVX group. n = 6. LPS (10 μg/mL) was used as an activator of TLR signaling pathway in the following experiments. b, c Relative mRNA expression of HMGB-1 and HMGB-1 protein were detected through qRT-PCR and western blotting, respectively. d Expression of TLR signaling pathway-related proteins TLR4, MyD88 p-P65 and P65 was detected by western blot. e Expressions of osteoclast marker genes OSCAR and TRAP were detected by western blot. f Trabeculae in all rats were visualized by H&E staining, Masson’s trichrome staining and Alcian blue staining. g The proportion of TRAP⁺ cells was shown. h The area ratio of cartilage was calculated. i The expressions levels of OSX, COL1A1 and DLX2 were measured with western blotting. j The levels of TNF-ɑ and IL-4 were measured via Elisa assays. *P < 0.05, vs WT OVX group; ^#P < 0.05, vs LPS + WT OVX group. n = 6. Data are from three biologically independent experiments