Table 1 Primer sequences for collagen Type I, collagen Type II, aggrecan and GAPDH
Target gene | Forward primer (5′–3′) | Reverse primer (3′–5′) |
|---|---|---|
Collagen type I | 5′ AAGAAGAAGACATCCCACCAGTC 3′ | 3′ ACAACACGCTACTGCACTAGA 5' |
Collagen type II | 5′ CCCGAACCCACAAACAACA 3′ | 3′ CCGAGACGTGACTTACCGA 5' |
Aggrecan | 5′ CCTACGATGTCTACTGCTATGTGG 3′ | 3′ CTTAGAGTATTGCGGTGGGAC 5' |
GAPDH | 5′ ATGTTTGTGATGGGCGTGAA 3′ | 3′TGACGAACCGAGGAGATCGG 5' |