Fig. 4

miRNA-4701-5p enhanced IL-1β-stimulated CHON-001 cells viability by down-regulating HMGA1. CHON-001 cells were transfected with mimic control, miRNA-4701-5p mimic, control-plasmid, HMGA1-plasmid for 24 h, respectively. Then the cells were induced by 10 ng/ml IL-1β for 12 h. A qRT-PCR analysis of miRNA-4701-5p expression. B, C Western blot assay and qRT-PCR analysis of HMGA1 expressions in different groups. D CHON-001 cells proliferation ability was detected by MTT assay. E The cytotoxicity of CHON-001 cells was accessed by LDH quantification. **p < 0.01 versus control; ^##p < 0.01 versus IL-1β + mimic control; ^&&p < 0.01 versus IL-1β + miRNA-4701-5p mimic + control-plasmid