Fig. 2
From: Cyclic mechanical strain with high-tensile triggers autophagy in growth plate chondrocytes
GPCs autophagy was activated by CTS in a magnitude- and time-dependent manner. The protein levels of LC3-II, LC3-I, ULK1, ATG5 and BECN1 were measured by western blotting. GAPDH was used as an internal control and served to normalize the results. A Chondrocytes were subjected to CTS at different magnitudes for 2 h. B–D Chondrocytes were subjected to CTS at 5500 με for 0.5 h, 2 h and 6 h. Error bars represent standard deviations. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001