Fig. 2

HuR silencing prevented osteogenic differentiation of MC3T3-E1 cells while promoting cell apoptosis. a RT-qPCR detection of the silencing efficiency of siHuR treatment. b RT-qPCR detection of osteoblast-related markers in MC3T3-E1 cells with HuR silencing. c Detection of ALP activity by p-nitrophenol assay in MC3T3-E1 cells with HuR silencing. d Detection of cell viability by MTT assay in MC3T3-E1 cells with HuR silencing. e Detection of the number of calcium nodules in MC3T3-E1 cells with HuR silencing by Alizarin red S staining. f Western blot determination of of osteogenic differentiation-related proteins (Runx2 and Osterix) in MC3T3-E1 cells with HuR silencing. g Detection of cell apoptosis in MC3T3-E1 cells with HuR silencing by TUNEL staining. OM, osteogenic medium. *p < 0.05 versus the NC group. Measurement data were expressed as mean ± standard deviation. Data between two groups were compared using unpaired t-test. Statistical analysis in relation to time-based measurements within each group was realized using repeated measures ANOVA, followed by a Bonferroni’s post hoc test. The cellular experiment was repeated three times independently