Fig. 3

Effects of circ_0001017 on the proliferation, migration and invasion of OS cells. Quantitative real-time PCR was utilized to analyze the expression of circ_0001017 in U2OS cells after transfection with circ_0001017 overexpression plasmid (A) or si-circ_0001017 (B). C MTT assay was used to assess cell proliferation of transfected U2OS cells. (D) Cell apoptosis of U2OS cells was determined by flow cytometry. E, F Transwell assay was applied to determine the cell migration and invasion capacities. Morphologic changes were examined under a fluorescence microscope at 200 × magnification. **p < 0.01, ***p < 0.001, compared with Vector; ##p < 0.01, ###p < 0.001, compared with si-NC