Fig. 4

Knockdown of circ_0001174 inhibits osteosarcoma (OS) cell proliferation, migration, and invasion. A Expression of circ_0001174 was determined using qRT-PCR in OS cell lines and control cells (hFOB1.19 cells). B Circ_0001174 expression level was detected after transfection with siRNA in MG63 and HOS cells. C Proliferation activity of si-circ_0001174 and si-NC MG63 cells was determined using CCK-8 assay. D Colony formation assay was performed to analyze the proliferation ability of stably transfected MG63 cells. E Transwell migration and invasion assays were used to measure the migration and invasion abilities of stably transfected cells. F Si-circ_0001174 suppressed cell migration capacity in the wound healing assay. *P < 0.05 and **P < 0.01