Fig. 3

Sox9 is a target gene of miR-539-3p. A Alignment of miR-539-3p and the 3′-UTR of Sox9, a potential miR-539-3p target. B A luciferase reporter carrying the 3′-UTR of wild-type (Sox9 3′UTR-WT) or mutant (Sox9 3′UTR-MUT) Sox9 was introduced into HEK-293T cells along with NC mimic or miR-539-3p mimic. C, F, and I The expression level of Sox9 in hASCs was determined by RT-qPCR. D, E, G, H, J, and K The protein expression levelof Sox9 in hASCs was detected by western blot. β-actin expression levels were detected as an endogenous control. ^**P < 0.01 (VS. 0 Day/NC mimic/pcDNA-NC). The experiment was repeated at least 3 times independently. The data are presented as mean ± standard deviation (SD)