Fig. 2

CD44 knockdown in MG63 and U2OS cells in vitro. A Transfection efficiency of MG‑63 and U2OS cells was assessed by fluorescence microscopy. GFP, green fluorescent protein. Magnification, × 200. B Reverse transcription‑quantitative PCR analysis was used to assess the mRNA expression levels of CD44 in MG‑63 and U2OS cells after transfection for 24 h. **P < 0.01 versus si-NC group. C Western blot was used to assess CD44 expression levels in MG‑63 and U2OS cells 48 h after transfection