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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Circ_0043947 contributes to interleukin 1β-induced injury in chondrocytes by sponging miR-671-5p to up-regulate RTN3 expression in osteoarthritis pathology

Fig. 3

Circ_0043947 acts as a molecular sponge for miR-671-5p. A The miRNA targets of circ_0043947 were predicted by bioinformatics algorithms using starbase and circinteractome databases. B The putative binding sites with circ_0043947 in miR-671-5p were shown. C Overexpression efficiency of miR-671-5p mimics in HEK293T cells was determined by RT-qPCR. D Dual-luciferase reporter assay was applied to verify the binding relation between circ_0043947 and miR-671-5p. E RT-qPCR assay was conducted to analyze the overexpression efficiency of miR-671-5p in chondrocytes. F RIP assay was performed to confirm the target relation between circ_0043947 and miR-671-5p. G The effect of circ_0043947 silencing on the expression of miR-671-5p was analyzed by RT-qPCR assay. H The expression of miR-671-5p was examined in IL-1β-induced chondrocytes (10 ng/mL, 24 h) by RT-qPCR assay. **P < 0.01, ***P < 0.001

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X