Fig. 3

Juglans regia L. extract enhanced cell autophagy of hBMSCs. hBMSCs were treated with OIM (osteogenic induction medium, including 10 mmol/L β-glycerophosphate, 10 nmol/L dexamethasone, and 10 μg/mL ascorbic acid) and different concentrations of Juglans regia L. extract (10, 20, 40, or 80 μM) for 7 days. The protein levels of LC3II (a and b), LC3I (a and b), Beclin-1 (a and d), and p62 (a and c) were tested by Western blot analysis. β-actin is a loading control. Data were represented as mean ± SD from at least three independent experiments performed for each assay. ***P < 0.001 vs control group