Fig. 7

MiR-516b-5p regulated Rg3-treated OS cell malignancy through KPNA4. A–G Rg3-treated MG63 cells and Saos2 cells were transfected with anti-miR-NC, anti-miR-516b-5p, anti-miR-516b-5p + si-NC, anti-miR-516b-5p + si-KPNA4, and cell viability was investigated by CCK-8 (A), cell proliferation by EdU assay (B), cell apoptosis by flow cytometry analysis (C), cell migration by wound-healing assay (D), cell invasion by transwell invasion assay (E), and the protein expression of PCNA, cleaved caspase-3 and MMP9 by Western blotting (F, G). *P < 0.05, **P < 0.01 and ***P < 0.001