Fig. 4

Circ_0003074 combined with miR-516b-5p. A The schematic illustration showing the complementary sites of circ_0003074 with miR-516b-5p. B The efficiency of miR-516b-5p overexpression was determined by qRT-PCR. C–F Dual-luciferase reporter and RNA pull-down assays were employed to verify the interplay between circ_0003074 and miR-516b-5p. G, H MiR-516b-5p expression was detected by qRT-PCR in the both MG63 cells and Saos2 cells treated with Rg3 at a dose of 0, 40, 80 or 120 µM. I, J MiR-516b-5p expression was checked by qRT-PCR in hFOB1.19 cells, MG63 cells, Saos2 cells, OS tissues (N = 38) and adjacent normal tissues (N = 38). K Spearman correlation analysis was used to predict the correlation of circ_0003074 and miR-516b-5p in OS tissues. L The effect of circ_0003074 overexpression on miR-516b-5p expression was revealed by qRT-PCR. M qRT-PCR was performed to determine the impact of miR-516b-5p on circ_0003074-induced downregulation of miR-516b-5p. **P < 0.01 and ***P < 0.001