Fig. 3

MiR-338-3p is a target of circ_0058058. A The putative binding site between circ_0058058 and miR-338-3p. B RT-qPCR analysis of miR-338-3p expression in cells transfected with miR-338-3p mimic or miR-NC. C, D Dual-luciferase reporter assay for the luciferase activity of wild and mutated circ_0058058 reporter after miR-338-3p overexpression in H929 and MM.1S cells. E, F Anti-Ago2 RIP assay was used in H929 and MM.1S cells to determine circ_0058058 and miR-338-3p RNA enrichment in immunoprecipitate complexes. G, H RT-qPCR analysis of miR-338-3p expression level in bone marrow aspirates of MM patients and normal healthy donors, as well as in MM cell lines (H929, OPM2 and MM.1S) and normal nPCs cells. I Transfection efficiencies of miR-338-3p inhibitor or inhibitor NC. J, K RT-qPCR analysis of miR-338-3p expression level in H929 and MM.1S cells transfected with si-NC, si-circ_0058058, si-circ_0058058 + anti-miR-NC, or si-circ_0058058 + anti-miR-338-3p