Fig. 2

Knockdown of circ_0058058 suppresses MM cell proliferation, angiogenesis and metastasis in vitro. A–K H929 and MM.1S cells were transfected with si-circ_0058058 or si-NC. A RT-qPCR analysis of circ_0058058 expression in cells. Cell proliferation analysis using CCK-8 (B, C), colony formation (D), and EdU assays (E). F Flow cytometry for cell apoptosis. G Tube formation ability of HUVECs cultivated for 6 h in the TCM from transfected H929 and MM.1S cells. H, I Transwell assay for cell migration and invasion. J, K Western blot analysis of the protein levels of E-cadherin, vimentin and N-cadherin. *P < 0.05