Fig. 3
From: Investigation of the role of the miR17-92 cluster in BMP9-induced osteoblast lineage commitment
miR-17 inhibits BMP9-induced ectopic bone formation in vivoand in vitro. Sponge miR-17 rescued down-regulated ALP activity at day 3,5, and 7. (A) and calcium deposit at day 14 (B) in BMP9-induced miR-17 iMEFs. C H & E (a) and Masson’s Trichrome staining (b) of the bone masses retrieved at week 4. B, mature bone; M, undifferentiated MSCs. iMEF cells were co-transfected with the luciferase reporters carrying 3′UTR of wild-type or mutated Rb1 (D), BMPRII (E) and SMAD7 (F). G Expression levels of miR-17 and RB1 in BMP9-induced osteogenic differentiation of MSCs