Fig. 2

The osteogenic effect of miR-17-92 cluster on BMP9-induced osteogenesis in vitro and in vivo. A The individual member of miR-17-92 cluster in BMP9-induced ALP activity in MSCs. Sub-confluent miR-17, miR-18a, miR-20a and miR-92a-1 iMEFs were infected with AdBMP9 or AdGFP at day 3 (a, b) and day 7 (c, d). Histochemical staining (a, c) and quantitative bioluminescence assay (b, d). Each assay was done in triplicate. **p < 0.01. B The function of individual member of miR-17-92 cluster in BMP9-induced calcium deposit. The infected cells were fixed and subjected to Alizarin Red S staining at day 14. Microscopic images (10 ×) are shown. C H & E (a) and Masson’s Trichrome staining (b) of the bone masses retrieved at week 4. B, mature bone; M, undifferentiated MSCs