Effects of LMHFV on the GSK3β/β-catenin pathway and related protein expression in HG-induced MC3T3-E1 cells. A, B Western blotting assays were performed to detect the protein expression of GSK3β, p-GSK3β, β-catenin, C-myc, Cyclin D1 and Runx2. The results are presented as the western blotting bands. C The grey value of the western blot band of each group was calculated, and the relative protein expression was compared, as presented in the bar graphs. *P<0.05, compared with the NC group. #P<0.05, compared with the HG group.