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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Overexpression of FGF2 delays the progression of osteonecrosis of the femoral head activating the PI3K/Akt signaling pathway

Fig. 3

The apoptosis assay revealed that DEX promoted cell apoptosis in MC3T3-E1 cells. a Effects of DEX on MC3T3-E1 cell proliferation, as demonstrated by CCK-8 assay. b Protein levels of Bax, Bcl-2, Cleaved caspase-3, Caspase-3 and FGF-2 were detected by western blotting after DEX treatment. c Flow cytometry after Annexin V/PI staining and quantitative analysis of the apoptosis ratio after DEX treatment. d Apoptosis by TUNEL staining after DEX treatment (× 100 magnification); *P < 0.05, DEX dexamethasone

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