Table 1 Sequences of primers used for quantitative real-time PCR
From: Protective role of the alpha-1-antitrypsin in intervertebral disc degeneration
Gene | Forward (5–3′) | Reverse (5–3′) |
|---|---|---|
Bax | AACTGGGGTCGATTGTG | GATCCAAGGCTCTAGGTGGTC |
Bcl-2 | TTGAGTTCGGTGGGGTCATG | GATCCAGGTGTGCAGATGCC |
Cleared Caspase-3 | TATGGAATTGATGGATAGT | CTGAAGAAACTAGTTAGTT |
Aggrecan | TCCAAACCAACCCGACAAT | TCTCATAGCGATCTTTCTTCTGC |
COL2A1 | ACGCTCAAGTCGCTGAACAA | TCAATCCAGTAGTCTCCGCTCT |
Collagen II | GGGAATGTCCTCTGCGATGAC | GAAGGGGATCTCGGGGTTG |
MMP-13 | CCGAGGAGAAACAATGATCT | GCCTGTATCCTCAAAGTGAA |
ADAMTS-5 | CGACAAGAGTCTGGAGGTGAG | CGTGAGCCACAGTGAAAGC |
Wnt1 | GAATCGCCGCTGGAACTGTC | GCGGAGGTGATAGCGAAGATAAACG |
β-catenin | CAAGTGGGTGGTATAGAGG | AGTCCATAGTGAAGGCGAAC |
GAPDH | TCAAGAAGGTGGTGAAGCAGG | TCAAAGGTGGAGGAGTGGGT |