Fig. 4
From: Hsa_circ_0004674 promotes osteosarcoma doxorubicin resistance by regulating the miR-342-3p/FBN1 axis
Hsa_circ_0004674 regulated OS DXR resistance by sponging miR-342-3p. A The transfection efficiency of miR-342-3p inhibitor was confirmed by detecting miR-342-3p expression in U2OS_R and KHOS_R cells using qRT-PCR. B MiR-342-3p expression was detected by qRT-PCR. C The DXR resistance of cells was assessed by CCK8 assay. The number of colonies, cell cycle process and apoptosis rate of cells were determined using colony formation assay (D) and flow cytometry (E–F). G WB analysis was performed to examine p-gp and MCL-1 protein levels. *P < 0.05