Fig. 3

X-ray analysis of femurs of older and younger mice on day 28 after infection. On day 28 after the intravenous bacterial inoculation, mice were euthanized and anteroposterior (AP) X-rays were obtained on the operative (op) and non-operative (non-op) legs of older and younger mice. a Representative AP radiographs. Yellow brackets denote femur width and blue brackets denote femur length. b Mean femur length measured perpendicular from the line bisecting the 3rd trochanter to the trochlea (mm) ± s.e.m.. c Mean femur width measured proximal to the fabella (mm) ± s.e.m.. *P < 0.05, †P < 0.01, and ‡P < 0.001, between op versus non-op legs in older and younger mice, as calculated by 2-tailed Mann Whitney U test (b, c). n.s. not significant