Fig. 3

CircMAPK9 directly interacted with miR-140-3p. A The putative binding sites between circMAPK9 and miR-140-3p were predicted by CircInteractome. B Dual-luciferase reporter assay was performed in RA-FLSs with co-transfection of circMAPK9 WT or circMAPK9 MUT and miR-NC or miR-140-3p. C The expression of miR-140-3p was examined by qRT-PCR in synovial tissues from RA patients (n = 22) and normal patients (n = 22). D The level of miR-140-3p was measured in H-FLSs and RA-FLSs by qRT-PCR. E The enrichment of miR-140-3p was analyzed via qRT-PCR in RA-FLSs with transfection of si-NC or si-circMAPK9#2. *P < 0.05