Fig. 6

CXCL12 overexpression alleviates miR-107 or silencing HOTAIR-mediated chondrocyte progression. A Cell proliferation was measured in chondrocytes transfected with miR-NC, miR-107 mimic, miR-107 mimic + pcDNA or CXCL12 overexpression vector by CCK-8 at 0, 24, 48 or 72 h. B Cell apoptosis was detected in chondrocytes transfected with miR-NC, miR-107 mimic, miR-107 mimic + pcDNA or CXCL12 overexpression vector by flow cytometry at 72 h after culture. C The protein levels of Aggrecan, Collagen II, MMP-13 and MMP-9 were measured in chondrocytes transfected with miR-NC, miR-107 mimic, miR-107 mimic + pcDNA or CXCL12 overexpression vector by western blot at 72 h after culture. D Cell proliferation was measured in chondrocytes transfected with si-NC, si-HOTAIR, si-HOTAIR + pcDNA or CXCL12 overexpression vector by CCK-8 at 0, 24, 48 or 72 h. E Cell apoptosis was detected in chondrocytes transfected with si-NC, si-HOTAIR, si-HOTAIR + pcDNA or CXCL12 overexpression vector by flow cytometry at 72 h after culture. F The protein levels of Aggrecan, Collagen II, MMP-13 and MMP-9 were measured in chondrocytes transfected with si-NC, si-HOTAIR, si-HOTAIR + pcDNA or CXCL12 overexpression vector by western blot at 72 h after culture. n = 3. Data were expressed as mean ± S.D. *P < 0.05