Fig. 4

GAS2 is a target gene of miR-520c-3p. a Putative binding sites between miR-520c-3p and GAS2. b The mRNA expression level of GAS2 in normal and OA cartilage. c Relative miR-520c-3p expression in normal and IL-1β-stimulated chondrocytes at 6, 12, 24, 36, and 72 h. d Relative miR-520c-3p expression in different concentration IL-1β-stimulated chondrocytes. e Correlation analysis between miR-520c-3p expression and GAS2 expression in normal and OA cartilage. qRT-PCR was applied to assess the mRNA level of GAS2 in control, IL-1β, IL-1β + NC mimic, and IL-1β + miR-520c-3p mimic groups. f Western blot assay and quantitative analysis the gray value of GAS2 expression in chondrocytes that treated with IL-1β, IL-1β + NC mimic, and IL-1β + miR-520c-3p mimic