Fig. 2

Ang II increased RANKL expression in synovial cells. Synovial cells were cultured in triplicate in the presence or absence of Ang II for the indicated times. Data are representative images or expressed as the mean ± SD of three independent experiments with synovial cells from each RA patient. a Viability of cells exposed to different concentrations of Ang II was determined by CCK8 assay (n = 3). b Western blot analysis of RANKL expression in cells exposed to different concentrations of Ang II (n = 3). c Western blot analysis of RANKL expression at different times (n = 3). *p < 0.05 vs. the control group