Fig. 5

CircASH2L indirectly regulates HIPK2 via miR-129-5p in RA-FLSs. a Binding sites of miR-129-5p on HIPK2 3′UTR. b Interaction analysis between miR-129-5p and HIPK2 using the dual-luciferase reporter assay. c-f HIPK2 expression level in synovial tissues with RA and non-RA, as well as in RA-FLSs and N-FLSs was determined. g MiR-129-5p expression analysis using qRT-PCR in RA-FLSs transfected with miR-NC or miR-129-5p. h, i HIPK2 expression analysis in miR-129-5p-increased RA-FLSs with qRT-PCR and western blot. j, k RA-FLSs were co-transfected with si-circASH2L and/or anti-miR-129-5p and HIPK2 level was examined using qRT-PCR and western blot. *P<0.05