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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: CircASH2L facilitates tumor-like biologic behaviours and inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2 axis in rheumatoid arthritis

Fig. 2

Effects of circASH2L on the tumor-like biologic behaviors of RA-FLSs. a-i si-circASH2L or si-NC was transfected into RA-FLSs, respectively. a Measurement of circASH2L expression in RA-FLSs with qRT-PCR. b, c Cell proliferation analysis with MTT assay and Edu assay. d Flow cytometry for cell apoptosis. e Detection of apoptosis-related protein using western blot. f Flow cytometry of cell cycle analysis. g, h Cell migration and invasion analysis with transwell assay. i Detection of inflammatory cytokines expression using ELISA. *P<0.05

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