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Table 2 The primers and siRNAs used in this study

From: Blocking circ-CNST suppresses malignant behaviors of osteosarcoma cells and inhibits glycolysis through circ-CNST-miR-578-LDHA/PDK1 ceRNA networks

Name

Sequence

circ-CNST (152 nt)

5′-TGTGCAACCACATACAGTTACG-3′ (forward)

5′-CAGCATATGTGACTGC-3′ (reverse)

linear CNST (165 nt)

5′-TCCCCTTTGCCTTCATCAGA-3′ (forward)

5′-TGTGGTTGCACAGTTTTCCA-3′ (reverse)

miR-578

5′-GTGCAGGGTGTTAGGA-3′ (forward)

5′-GAAGAACACGTCTGGT-3′ (reverse)

LDHA (165 nt)

5′-CAGGTGGTTGAGAGGGTCTTT-3′ (forward)

5′-CTTCAAACGGGCCTCTTCCT-3′ (reverse)

PDK1

5′-GCTTCGATGCAGCAACAACA-3′ (forward)

5′-AGGCGCTGCTTTAAGCTCTG-3′ (reverse)

β-actin

5′-ACACCTTCTACAATGAGCTG-3′ (forward)

5′-CTGCTTGCTGATCCACATCT-3′ (reverse)

U6

5′-CTCGCTTCGGCAGCACAT-3′ (forward)

5′-AACGCTTCACGAATTTGCGT-3′ (reverse)

si(sh)-circ-CNST

5′-GTCTCTTGATGTGTTGCACAA-3′ (sense)

5′-GTGCAACACATCAAGAGACTT-3′ (anti-sense)

si (sh)-NC

5′-TCTGGTTGACATCATGCTTAATGTG-3′ (sense)

5′-CATTAAGCATGATGTCAACCAGACA-3′ (anti-sense)

miR-578 mimic

5′-CUUCUUGUGCUCUAGGAUUGU-3′

miR-NC

5′-GGUUCGUACGUACACUGUUCA-3′

anti-miR-578

5′-ACAATCCTAGAGCACAAGAAG-3′

anti-miR-NC

5′-UGAACAGUGUACGUACGAACC-3′