Fig. 6

The downstream targets of miR-578 in human OS cells. a, b Alignment sequences between miR-578 and LDHA 3′UTR-WT/MUT or PDK1 3′UTR-WT/MUT. c, d Dual-luciferase reporter assay identified relative luciferase activity of 143B and U2OS cells co-transfected with WT/MUT vector and miR-578 or miR-NC. e, g RT-qPCR detected relative LDHA and PDK1 mRNA expression in tumor tissues from OS patients (n = 29) comparing to adjacent normal tissues. f, h Pearson correlation coefficient analysis validated the linear correlation between miR-578 expression and mRNA expression of LDHA or PDK1 in human OS tumors. i–l Western blotting measured relative protein expression of LDHA and PDK1 in OS patients’ tumor tissues and adjacent tissues, and cell lines including 143B, U2OS cells, and hFOB1.19 cells. **P < 0.01, ***P < 0.001, and ****P < 0.0001